A Neuroscience Field Guide: Nerve Growth Factor

Nerve growth factor, NGF for short, is a soluble protein that is secreted by various tissues in the body, and it promotes the growth of nerve cell processes and survival of neurons. It is the first of a class of molecules known as neurotrophins which are very important for the development and function of the nervous system.

What is remarkable about NGF is how it was discovered and by whom. NGF was discovered by Rita Levi-Montalcini an Italian, Jewish young doctor. She originally became interested in a set of experiments by renowned embryologist Viktor Hamburger which had observed that removing a limb bud from a chicken embryo caused the sensory neurons that innervated the undeveloped limb to die off. This suggested that there was something about the target tissue that promoted neurons’ survival in the embryo.

Levi-Montalcini had just graduated from medical school when Mussolini issued the “Manifesto per la Difesa della Razza”, which ultimately led to a ban of all non-Aryans from having professional and academic careers. Undaunted, Levi-Montalcini set up a laboratory in her bedroom in her parents house and then after the bombing of Turin in her family’s country cottage. After the War she was invited to St. Louis, MO to join Viktor Hamburger where she remained for many years and where she performed her Nobel Prize-winning research.

One of her initial observations was that if you implant specific mouse tumor cells on a chick embryo, sensory neurons will grow rapidly and sprout new axons which will innervate the tumor cells, again confirming that certain target tissues can promote nerve growth in embryonic tissues. The question was how did the target tissues do this? The key experiment came when she and her colleagues grew on one end of a cell culture dish some of these mouse tumor cells, and on the other a bit of neural tissue known as a sensory ganglion. After a few days, she observed that the neurons in the sensory ganglia grew a bunch new axons, and these axons seemed to be oriented toward the tumor cells. As if they were being attracted. This told her that the tumor cells were actually releasing some soluble factor into the growth media. The factor promoted growth of nerve cells and helped them live longer in a culture dish. This factor was later isolated by biochemist Stanley Cohen and shown to be a protein which they dubbed, imaginatively, nerve growth factor. Both Cohen and Levi-Montalcini received Nobel Prize in 1986.

 

From Levi-Montalcini’s Nobel Lecture: Drawing of the cell culture experiment. The tissue on the left is the mouse tumor cells, the tissue on the left is the sensory ganglion. Note the axons growing out towards the mouse tissue.

 

How does NGF work? Now we know that NGF, as well as several other similar proteins known as neurotrophins, activate receptors in target cells which are called tyrosine receptor kinases, or Trk. Trks are proteins which are on the surface of neurons that when activated by NGF cause a series of cellular processes which cause embryonic neurons to grow new axons and to survive. Any cells which do not find their target will undergo a self-destructive process called apoptosis, thus help in the developing nervous system keep appropriate connections and eliminate inappropriate ones. Furthermore, NGF could form the basis of new therapies for treating various degenerative brain disorders and maybe promote neural regeneration after injury.

Rita Levi-Montalcini is now 103 years old, the oldest living Nobel laureate. Apparently she uses NGF eye-drops daily (it’s true!).

Rita Levi-Montalcini in 2009, at her 100th birthday party.

 

Further Reading

Rita Levi-Montalcini Nobel Lecture and her Autobiography

Stanley Cohen Nobel Lecture

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7 Responses to A Neuroscience Field Guide: Nerve Growth Factor

  1. anon says:

    Thanks for this. Dr. Levi-Montalcini is a superhero.

  2. They’re called neurotrophins, not neurotropins.

    • namnezia says:

      Crap, I always misspell that! Thanks for pointing it out.

      • It’s more than just a misspelling. Tropism is something different from trophism, and, in fact, you can see tropism in RLM’s drawing from her Nobel lecture as well as trophism.

      • personwhoisinterestedinscience says:

        just because they misspelled something important doesnt mean they did it on purpose
        thankyou so much for this it helped me with a very important science assignment

  3. Hi, just wanted to say, I enjoyed this blog post. It was funny. Keep on posting!

  4. www.peaknootropics.com says:

    Thanks for this post. I have been heavily interested in using NGF as a nootropic. I wanted to learn more about NGF and stumbled upon your post and it gave me a much more detailed explanation that was easier to understand than Wikipedia.

    Anyway, I wanted to note that Hericium erinaceus also know as Lion’s Mane Mushroom is a source for natural NGF. I am planning on adding this to my “brain stack” which includes a long list of nutrients and racetam nootropics as well as supplements to promote LTP (long term potentiation – a theory behind synaptic plasticity)

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